Journal: PLoS ONE

Article Title: Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach

doi: 10.1371/journal.pone.0254739

Figure Lengend Snippet: (A) The FabFluor-488 positive area was normalized to the phase contrast area over time after transduction. The IgG1 isotype negative control and the lentiviral vector (LV) negative control (matrix) showed no FabFluor488 signal. The anti-transferrin-receptor IgG1 positive control antibody confirmed successful FabFluor488 labeling of the test antibodies. Matrix control overlaps with the negative control. Data represent the mean ± standard deviation of three technical replicates. (B) Normalized FabFluor488 peak value vs. time at which this value is reached. The datapoint in the upper left with the highest normalized positive area represents the 1:2 LV dilution. The other datapoints represent two-fold serially diluted LV up to a dilution of 1:1024 with the lowest normalized positive area and the longest time after which the peak value is reached. Data represent the mean ± standard deviation of three biological replicates.

Article Snippet: A negative isotype control antibody (1 μg/mL) and a positive control anti-transferrin-receptor antibody (3 μg/mL) (R&D Systems), both a mouse IgG1 subtype, were labeled at a 1:3 molar ratio with FabFluor-488.

Techniques: Transduction, Negative Control, Plasmid Preparation, Positive Control, Labeling, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Cell sheets of co-cultured BMP-2-modified bone marrow stromal cells and endothelial progenitor cells accelerate bone regeneration in vitro

doi: 10.3892/etm.2019.7982

Figure Lengend Snippet: Characterization of BMSCs and EPCs. (A) Flow cytometry analysis of Ficoll-separated BMSCs surface markers, including CD44, CD71 and CD45. (B) Flow cytometry analysis of Percoll-separated EPCs surface markers, including CD31, CD133 and VEGFR. BMSCs, bone marrow stromal cells; EPCs, endothelial progenitor cells; CD44, CD44 antigen; CD71, transferrin receptor protein 1; CD45, receptor-type tyrosine-protein phosphatase C; CD31, platelet endothelial cell adhesion molecule; CD133, prominin-1; VEGFR, vascular endothelial growth factor receptor; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Article Snippet: Subsequently, primary antibodies purchased from BIOSS, including fluorescein isothiocyanate/phycoerythrin (FITC/PE)-conjugated rat anti-CD44 (cat. no. bs-4916R-FITC), FITC/PE-conjugated rat anti-CD71 (cat. no. bs-1782R-FITC) and FITC/PE-conjugated rat anti-receptor-type tyrosine-protein phosphatase C (CD45) (cat. no. bs-10599R-FITC) at a concentration of 2 mg/ml for the identification of BMSCs; and FITC/PE-conjugated rat anti-CD31 (cat. no. bs-0195R-FITC), FITC/PE-conjugated rat anti-CD133 (cat. no. bs-0395R-FITC) and FITC/PE-conjugated rat anti-VEGFR (cat. no. bs-0170R-FITC) were added to the cells at a concentration of 2 mg/ml and incubated with anti-mouse compensation beads for 30 min in the dark for the identification of EPCs.

Techniques: Flow Cytometry